Detection of plant RNA viruses by nonisotopic dot-blot hybridization. Polymerase chain reaction (PCR) provides a powerful technique for identifying viruses and studying the homology between viral nucleic acids. Dot-blot hybridization analysis of subtracted fragments membranes were screened by hybridization with genomic DNA from the tester (panel A) and the driver. The dot blot hybridization assay using the nonradioactive probe led to similar results to indirect immunofluorescence (IFI) when tested against a panel of. . Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. RNA slot-blot hybridization of two cotton cultivars, Giza 45 and Giza 84, performed by use of their respective related probes G45 an d G84 probes. Additional Online Courses. Slot Blot Hybridization · AluQuant · Quantitative PCR · Data Analysis for Quantitative PCR · Summary of Advantages and Disadvantages. Dot-blot hybridization method definition, Principle, steps, advantages A simplified 3 minute videoThe video explainsDot-blot hybridization. Fig.