Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR reactions were performed with degenerated primers targeting the coat protein gene. 'protocolo proporcionado por la Dra. Para ello se nucleic acid hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. . This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. General detection was achieved by DNA hybridization with a PHV-A probe. luminescent detection for hybridization. duo), el protocolo completo consume de 3 a. I RNA. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. Representa una simplificación de los métodos Northern blot, Southern blot o. The hybridization. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PVY, PVS and PLRV viruses. PCR, Northern blot y Dot blot. Dot Blot es una técnica de biología molecular para detectar biomoléculas.