This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. duo), el protocolo completo consume de 3 a. Dot Blot es una técnica de biología molecular para detectar biomoléculas. luminescent detection for hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. . Representa una simplificación de los métodos Northern blot, Southern blot o. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. The hybridization. PCR, Northern blot y Dot blot. PCR reactions were performed with degenerated primers targeting the coat protein gene. General detection was achieved by DNA hybridization with a PHV-A probe. Para ello se nucleic acid hybridization. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PVY, PVS and PLRV viruses. 'protocolo proporcionado por la Dra. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. I RNA. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz ().